Structural transitions in influenza haemagglutinin at membrane fusion pH
نویسندگان
چکیده
منابع مشابه
Analyses of the antigenicity of influenza haemagglutinin at the pH optimum for virus-mediated membrane fusion.
At the pH optimum for membrane fusion the haemagglutinin glycoprotein (HA) of the influenza virus membrane which is implicated in the fusion activity undergoes a conformational change. We have analysed the effects of this change on the antigenicity of the haemagglutinin by reacting the molecule with monoclonal antibodies of defined specificity. The results obtained indicate that specific change...
متن کاملFusion of influenza virus membranes with liposomes at pH 7.5.
Influenza virus X-31 (H3N2) membranes fuse with liposomes containing ganglioside GD1a at pH 7.5. Fusion was demonstrated by electron microscopy and also can be measured by counting the labeled virus proteins incorporated into liposomes after bound virus has been removed. Liposomes composed of lipids that have no net charge behave as reported by other investigators and do not fuse with influenza...
متن کاملpH-induced structural transitions in beta-lactoglobulin.
Pharmaceutique units/mg; Novo, Copenhagen, Denmark) and a-chymotrypsin (Choay, Paris, France) were, under appropriate conditions, added to the partially purified enzyme in final concentrations of 50 and 250 pg/ml and incubated at 37°C for different time intervals, ranging from 1 to 24 h. In all proteinase-treated preparations the DPP IV activity measured exceeded 90% of the original DPP IV acti...
متن کاملStructural insights into membrane fusion at the endoplasmic reticulum.
T he endoplasmic reticulum (ER) in eukaryotes is composed of an interconnected network of membrane tubules that undergoes permanent membrane fusion and fission. A hallmark of this membrane system is the presence of so-called three-way junctions (Fig. 1). These connections between adjacent membrane tubules are essential for the continuity of the ER luminal space. In 2009, two groups identified t...
متن کاملThe pH sensor for flavivirus membrane fusion
Viruses that infect cells by uptake through endosomes have generally evolved to "sense" the local pH as part of the mechanism by which they penetrate into the cytosol. Even for the very well studied fusion proteins of enveloped viruses, identification of the specific pH sensor has been a challenge, one that has now been met successfully, for flaviviruses, by Fritz et al. (Fritz, R., K. Stiasny,...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Nature
سال: 2020
ISSN: 0028-0836,1476-4687
DOI: 10.1038/s41586-020-2333-6